Melanopsin-SAP References

Cat #IT-44

2018

Garrett AM, Khalil A, Walton DO, & Burgess RW. DSCAM promotes self-avoidance in the developing mouse retina by masking the functions of cadherin superfamily members. (2018). Proc Natl Acad Sci, 115 (43):E10216-E10224.

Summary: focus on DSCAM (Down syndrome cell adhesion molecule 1) self-avoidance function in the mouse retina. DSCAM and members of the cadherin superfamily have also emerged as key contributors to a variety of neurodevelopmental disorders, including autism, schizophrenia, bipolar disease, Down syndrome and intellectual disability.

Ostrin LA, Strang CE, Chang K, Jnawali A, Hung L-F, Arumugam B, Frishman LJ, Smith EL, & Gamlin PD. Immunotoxin-Induced Ablation of the Intrinsically Photosensitive Retinal Ganglion Cells in Rhesus Monkeys. (2018). Front Neurol, 9 1000.

Objective:  To develop and validate a targeted ipRGC immunotoxin to ultimately examine the role of ipRGCs in macaque monkeys.
Summary:  Findings demonstrated that Melanopsin-SAP was specific for ipRGCs, and induced a graded reduction in the PLR (pupillary light reflex), as well as in ipRGC-driven pupil response with concentration.
Dose:  Solutions of 0.316, 1, 3.16, 10, and 50 μg delivered intravitreally.

Potter H, Alenciks E, Frazier K, Porter A, & Fraley GS. Immunolesion of Melanopsin Neurons Causes Gonadal Regression in Pekin Drakes (Anas Platyrhynchos Domesticus). (2018). General and Comparative Endocrinology, 256 16-22.

Objective:  Examine effects of loss of melanopsin in drakes.
Summary:  Loss of melanopsin in PMM elicits decrease in GnRH mRNA expression, gonadal regression, and sex behaviors in drakes.
Dose:  To specifically lesion melanopsin-receptive neurons, 3 μl of an anti-melanopsin-saporin conjugate (MSAP, 100 ng/ul) was injected into the lateral ventricle (n = 10). Control drakes were injected with 3 μl of equimolar unconjugated anti-melanopsin and saporin (SAP, n = 10).

2017

Huang L, Yuan T, Tan M, Xi Y, Hu Y, Tao Q, Zhao Z, Zheng J, Han Y, Xu F, Luo M, Sollars PJ, Pu M, Pickard GE, So KF, & Ren C. A retinoraphe projection regulates serotonergic activity and looming-evoked defensive behaviour. (2017). Nat Commun, 8 14908. 2017/04/01. PMC5381010

Objective:  To investigate how the dorsal raphe nucleus (DRN) and superior colliculus work in concert to extract and translate visual threats into defensive behavioural responses.
Summary:  A dedicated population of RGCs signals rapidly approaching visual threats and their input to the DRN controls a serotonergic self-gating mechanism that regulates innate defensive responses.
Dose:Mice received bilateral intraocular injection (2 μg per eye) made between Streptavidin-Saporin and a biotinylated CTB antibody, or Anti-Melanopsin-SAP (2 μg per eye).  For detection of melanopsin, retinas were incubated for 3 days at 4 °C with anti-melanopsin (1:600).

Zhang J, Wang H, Wu S, Liu Q, Wang N.  (2017) Regulation of Reentrainment Function Is Dependent on a Certain Minimal Number of Intact Functional ipRGCs in rd Mice. J Ophthalmol 2017:6804853. doi: 10.1155/2017/6804853

2013

Ren C, Luan L, Wui-Man Lau B, Huang X, Yang J, Zhou Y, Wu X, Gao J, Pickard GE, So KF, Pu M. (2013) Direct Retino-Raphe Projection Alters Serotonergic Tone and Affective Behavior. Neuropsychopharmacology 38(7):1163-1175 (Targeting Trends 13q2)

2011

See: Society for Neuroscience 2011 Abstracts

2010

Do MTH, Yau K (2010) Intrinsically photosensitive retinal ganglion cells. Physiol Rev 90(4):1547-1581. (Targeting Trends 11q1)

2009

Ingham ES, Gunhan E, Fuller PM, Fuller CA.  (2009) Immunotoxin-induced ablation of melanopsin retinal ganglion cells in a non-murine mammalian model. J Comp Neurol 516:125-140. doi: 10.1002/cne.22103

2008

Goz D, Studholme K, Lappi DA, Rollag MD, Provencio I, Morin LP (2008) Targeted destruction of photosensitive retinal ganglion cells with a saporin conjugate alters the effects of light on mouse circadian rhythms. PLoS ONE 3:e3153. (Targeting Trends 09q1)