Ostrin LA, Strang CE, Chang K, Jnawali A, Hung L-F, Arumugam B, Frishman LJ, Smith EL, & Gamlin PD. Immunotoxin-Induced Ablation of the Intrinsically Photosensitive Retinal Ganglion Cells in Rhesus Monkeys. (2018). Front Neurol, 9 1000.
Objective: To develop and validate a targeted ipRGC immunotoxin to ultimately examine the role of ipRGCs in macaque monkeys.
Summary: Findings demonstrated that Melanopsin-SAP was specific for ipRGCs, and induced a graded reduction in the PLR (pupillary light reflex), as well as in ipRGC-driven pupil response with concentration.
Dose: Solutions of 0.316, 1, 3.16, 10, and 50 μg delivered intravitreally.
Potter H, Alenciks E, Frazier K, Porter A, & Fraley GS. Immunolesion of Melanopsin Neurons Causes Gonadal Regression in Pekin Drakes (Anas Platyrhynchos Domesticus). (2018). General and Comparative Endocrinology, 256 16-22.
Objective: Examine effects of loss of melanopsin in drakes.
Summary: Loss of melanopsin in PMM elicits decrease in GnRH mRNA expression, gonadal regression, and sex behaviors in drakes.
Dose: To specifically lesion melanopsin-receptive neurons, 3 μl of an anti-melanopsin-saporin conjugate (MSAP, 100 ng/ul) was injected into the lateral ventricle (n = 10). Control drakes were injected with 3 μl of equimolar unconjugated anti-melanopsin and saporin (SAP, n = 10).
Zhang J, Wang H, Wu S, Liu Q, Wang N. (2017) Regulation of Reentrainment Function is Dependent on a Certain Minimal Number of Intact Functional Iprgcs in Rd Mice. J Ophthalmol .
Ren C, Luan L, Wui-Man Lau B, Huang X, Yang J, Zhou Y, Wu X, Gao J, Pickard GE, So KF, Pu M. (2013) Direct Retino-Raphe Projection Alters Serotonergic Tone and Affective Behavior. Neuropsychopharmacology 38(7):1163-1175 (Targeting Trends 13q2)
Do MTH, Yau K (2010) Intrinsically photosensitive retinal ganglion cells. Physiol Rev 90(4):1547-1581. (Targeting Trends 11q1)
Goz D, Studholme K, Lappi DA, Rollag MD, Provencio I, Morin LP (2008) Targeted destruction of photosensitive retinal ganglion cells with a saporin conjugate alters the effects of light on mouse circadian rhythms. PLoS ONE 3:e3153. (Targeting Trends 09q1)