SP-CTA References

Cat #IT-39

Zielinski MR, & Gerashchenko D. Sleep-inducing effect of substance P-cholera toxin A subunit in mice. (2017). Neurosci Lett, 659 44-47. 09/01.

Objective:  To determine the effects of selectively activating SP-expressing brain cells on sleep regulation in mice.
Summary:   ICV administration of SP-CTA produces increased amounts of NREM sleep but induces sleep fragmentation.
Dose:  1 mcg/mcl icv.

Caudle RM, King C, Nolan TA, Suckow SK, Vierck CJ, Jr., & Neubert JK. Central sensitization in the trigeminal nucleus caudalis produced by a conjugate of substance P and the A subunit of cholera toxin. (2010). J Pain, 11 (9):838-846. 2010/07/14. PMC2930122

Objective:  To understand central sensitization.
Summary:  Findings suggest that central sensitization leads to activation of an endogenous opioid system. Intracisternal administration of SP-CTA  is a useful model for studying central sensitization as a disease process without having to induce a peripheral injury.
Dose:  SP-CTA was diluted in PBS and injected in a volume of 10 μl (rats) or 1 μl (mice) over the course of three minutes.

Caudle RM. (2007) Inducing Central Sensitization with a Substance P/ Cholera Toxin Conjugate. Targeting Trends 8(4).

Caudle RM (2007) Sensitization of spinal cord nociceptive neurons with a conjugate of substance P and cholera toxin. BMC Neuroscience 8:30.