FGF-SAP References

Cat #IT-38

Vikan AK, Kostas M, Haugsten EM, Selbo PK, Wesche J (2021) Efficacy and Selectivity of FGF2-Saporin Cytosolically Delivered by PCI in Cells Overexpressing FGFR1. Cells 10(6):1476. doi: 10.3390/cells10061476

Summary: Fibroblast growth factor receptors (FGFRs) have become an attractive target in cancer research and therapy due to their implication in several cancers. The authors evaluated the efficacy and selectivity of PCI of FGF2-saporin (FGF-SAP) in cells overexpressing FGFR1. The authors conclude that to prevent off-target effects of FGF-based toxins, it will be necessary to circumvent binding to HSPGs, for example by mutating the binding site of FGF2 to HSPGs.

Esko JD, Stanley P. Glycosylation Mutants of Cultured Mammalian Cells (2017) In: Varki A, Cummings RD, Esko JD, Stanley P, Hart GW, Aebi M, Darvill AG, Kinoshita T, Packer NH, Prestegard JH, Schnaar RL, Seeberger PH, editors. Essentials of Glycobiology [Internet]. 3rd ed. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2015–2017. Chapter 49. doi: 10.1101/glycobiology.3e.049

Dickey DD, Thomas GS, Dassie JP, Giangrande PH. (2016) Method for Confirming Cytoplasmic Delivery of RNA Aptamers. Methods Mol Biol 1364:209-217. PMID: 26472453 (Targeting Trends 16q1)

Hernandez, LI, Flenker, KS, Hernandez, FJ, Klingelhutz, AJ, McNamara, JO, 2nd, & Giangrande, PH. (2013). Methods for Evaluating Cell-Specific, Cell-Internalizing Rna Aptamers. Pharmaceuticals (Basel), 6 (3):295-319. PMID: 23894227 (read summary)

Liao D, Lin PH, Yao Q, Chen C (2008) Vascular smooth cell proliferation in perfusion culture of porcine carotid arteries. Biochem Biophys Res Commun 372(4):668-673. (Targeting Trends 08q3)

Davol P, Frackelton AR Jr.  The mitotoxin, basic fibroblast growth factor-saporin, effectively targets human prostatic carcinoma in an animal model.  J Urol. 1996 Sep;156(3):1174-9. PMID: 8709341

Davol P, Beitz JG, Mohler M, Ying W, Cook J, Lappi DA, Frackelton AR Jr.  Saporin toxins directed to basic fibroblast growth factor receptors effectively target human ovarian teratocarcinoma in an animal model.  Cancer. 1995 Jul 1;76(1):79-85.   PMID:  8630880

Beitz JG, Davol P, Clark JW, Kato J, Medina M, Frackelton AR, Jr., Lappi DA, Baird A, Calabresi P (1992) Antitumor activity of basic fibroblast growth factor-saporin mitotoxin in vitro and in vivo. Cancer Res 52:227-230.

David T, Tassin J, Lappi DA, Baird A, Courtois Y (1992) Biphasic effect of the mitotoxin bFGF-saporin on bovine lens epithelial cell growth: effect of cell density and extracellular matrix. J Cell Physiol 153:483-490.

Gonzalez AM, Lappi DA, Buscaglia ML, Carman LS, Gage FH, Baird A (1991) Basic FGF-SAP mitotoxin in the hippocampus. Specific lethal effect on cells expressing the basic FGF receptor. Ann N Y Acad Sci 638:442-444.

Lindner V, Lappi DA, Baird A, Majack RA, Reidy MA (1991) Role of basic fibroblast growth factor in vascular lesion formation. Circ Res 68:106-113.

Beattie GM, Lappi DA, Baird A, Hayek A (1990) Selective elimination of fibroblasts from pancreatic islet monolayers by basic fibroblast growth factor-saporin mitotoxin. Diabetes 39:1002-1005.