CCK-SAP References

Cat #IT-31

2021

Han W, de Araujo IE (2021) Dissection and surgical approaches to the mouse jugular-nodose ganglia. STAR Protocols 2(2):100474. doi: 10.1016/j.xpro.2021.100474

Summary: For complete details on the use and execution of this protocol, please refer to Han et al. (2018)
Dose: Injected 0.5 µl of CCK-SAP (250 ng/µl) into the R-NG of VGlut2-ires-Cre mice.

Lee TH, Yau SY (2021) From Obesity to Hippocampal Neurodegeneration: Pathogenesis and Non-Pharmacological Interventions. Int J Mol Sci 22(1):201. doi: 10.3390/ijms22010201

Summary: This review provides insights into how chronic metabolic disorders, like obesity, could impair brain health and cognitive functions in later life. The authors reference the use of CCK-SAP  into the nodose ganglia to impair spatial memory and contextual episodic memory.   See Suarez AN, Hsu TM, Liu CM, Noble EE, Cortella AM, Nakamoto EM, Hahn JD, de Lartigue G, Kanoski SE. Gut vagal sensory signaling regulates hippocampus function through multi-order pathways. (2018) Nat Commun 9(1):2181. doi: 10.1038/s41467-018-04639-1

McDougle M, Quinn D, Diepenbroek C, Singh A, de la Serre C, de Lartigue G (2021) Intact vagal gut-brain signalling prevents hyperphagia and excessive weight gain in response to high-fat high-sugar diet. Acta Physiol (Oxf) 231(3):e13530. doi: 10.1111/apha.13530

Objective: To assess the function of the vagus nerve lack specificity.
Summary: Intact sensory vagal neurons prevent hyperphagia and exacerbation of weight gain in response to a HFHS diet by promoting lipid-mediated satiation.
Dose:  Rat nodose ganglia were injected bilaterally with either CCK-SAP or unconjugated saporin as a control.

2019

Suarez AN, Liu CM, Cortella AM, Noble EN, Kanoski SE (2019) Medial septum cholinergic signaling regulates gastrointestinal-derived vagus sensory nerve communication to the hippocampus. Neuroscience 2019 Abstracts 601.19. Society for Neuroscience, Chicago, IL

Tominaga M, Kusube F, Honda K, Komiya E, Takahashi N, Naito H, Suga Y, & Takamori K. OP11: Role of spinal cholecystokinin receptor 2 in alloknesis models. (2019) Itch 4:1-62. doi: 10.1097/itx.0000000000000030

Objective: To determine the detailed molecular and cellular mechanisms that induce alloknesis via the spinal CCK2 receptor.
Summary:  Ablation of spinal CCK receptor-expressing cells by i.t. injection of CCK-SAP attenuated CCK8S-induced alloknesis in comparison with Blank-SAP control mice.
Dose:  Intrathecal injection.

2018

Han W, Tellez LA, Perkins MH, Perez IO, Qu T, Ferreira J, Ferreira TL, Quinn D, Liu Z-W, Gao X-B, Kaelberer MM, Bohórquez DV, Shammah-Lagnado SJ, de Lartigue G, & de Araujo IE. A Neural Circuit for Gut-Induced Reward. (2018). Cell, 175 (3):665-678.

Objective:  To determine relevant gut-brain neuronal circuitry to motivational and emotional states.
Summary:  There is a critical role for the vagal gut-to-brain axis in motivation and reward.
Dose:  Injected 0.5 µl of CCK-SAP (250 ng/µl) into the R-NG of VGlut2-ires-Cre mice.

Suarez AN, Hsu TM, Liu CM, Noble EE, Cortella AM, Nakamoto EM, Hahn JD, de Lartigue G, Kanoski SE. Gut vagal sensory signaling regulates hippocampus function through multi-order pathways. (2018) Nat Commun 9(1):2181. doi: 10.1038/s41467-018-04639-1

Objective:  To determine the  endogenous relevance of GIderived vagal HPC communication.
Summary:  Endogenous derived vagal sensory signaling promotes HPC-dependent memory function via a multi-order brainstem–septal pathway, thereby identifying a previously unknown role for the gut–brain axis in memory control.
Dose:   A 1-µl volume of CCK-SAP (250 ng/µl) or control Saporin (250 ng/µl) was injected at two sites: 0.5 µl rostral and 0.5 µl caudal to the laryngeal nerve branch.

2017

Diepenbroek C, Quinn D, Stephens R, Zollinger B, Anderson S, Pan A, de Lartigue G. (2017) Validation and Characterization of a Novel Method for Selective Vagal Deafferentation of the Gut. Am J Physiol Gastrointest Liver Physiol 313(4):G342-52. PMID: 28705805

Objective:  To develop a new method that allows targeted lesioning of vagal afferent neurons that innervate the upper GI tract while sparing vagal efferent neurons.
Summary:  CCK-SAP ablates a subpopulation of VAN in culture. In vivo, CCK-SAP injection into the NG reduces VAN innervating the mucosal and muscular layers of the stomach and small intestine but not the colon, while leaving vagal efferent neurons intact.
DoseIn vitro: each well was treated with a different dose of saporin conjugates (0, 2.4, 24, or 240 ng) for 24 h.  In vivo: An equal volume (rat: 1 µl; mouse: 0.5 µl) of CCK-SAP (250 ng/µl) or Saporin (250 ng/µl) was injected at two sites rostral and caudal to the laryngeal nerve branch.

Suarez AN, Hsu TM, DeLartigue G, Kanoski SE (2017) Gastrointestinal vagal afferent signaling promotes hippocampal-dependent memory function in rats. Neuroscience 2017 Abstracts 510.22 / PP13. Society for Neuroscience, Washington, DC

2010

Datta S, Chatterjee K, Wiley R (2010) Decreasing abnormal nocifensive responses in the bilateral chronic constriction injury (bCCI) model of neuropathic pain: Effects of lumbar intrathecal CCK-saporin. Neuroscience 2010 Abstracts 175.22/MM12. Society for Neuroscience, San Diego, CA

2009

Datta S, Chatterjee K, Kline IV RH, Wiley RG (2009) CCK receptor- expressing dorsal horn neurons: Role in pain and morphine analgesia. Neuroscience 2009 Abstracts 265.13/Z37. Society for Neuroscience, Chicago, IL

Morin LP, Studholme KM (2009) Saporin lesions that target suprachiasmatic cells bearing NPY receptors eliminate or greatly impair circadian rhythm generation and entrainment. Neuroscience 2009 Abstracts 278.7/EE49. Society for Neuroscience, Chicago, IL

Zhang W, Gardell S, Zhang D, Xie JY, Agnes RS, Badghisi H, Hruby VJ, Rance N, Ossipov MH, Vanderah TW, Porreca F, Lai J (2009) Neuropathic pain is maintained by brainstem neurons co-expressing opioid and cholecystokinin receptors. Brain 132:778-787. (Targeting Trends 09q3)

2008

Datta S, Chatterjee K, Kline IV RH, Wiley RG (2008) Lumbar intrathecal CCK-saporin: anatomic and nociceptive effects. Neuroscience 2008 Abstracts 773.4/MM32. Society for Neuroscience, Washington, DC

Edelmayer RM, Vanderah TW, Majuta L, Fioravanti B, De Felice M, Chichorro JG, Ossipov MH, King T,Lai J, Kori SH, Nelsen AC, Cannon KE, Heinricher MM, Porreca F (2008) A brainstem generator for cutaneous allodynia associated with migraine headache. Neuroscience 2008 Abstracts 171.15/LL16. Society for Neuroscience, Washington, DC

2006

Lai J, Zhang W, Badghisi H, Hruby VJ, Porreca F. (2006) The Biologically Active Cholecystokinin (26-33) peptide, [Tyr2-SO3]CCK-8, Retains High Affinity for CCK2 Receptors after Covalent Conjugation to Saporin. Targeting Trends 7(1).

2005

Zhang W, Gardell SE, Xie Y, Luo M, Rance NE, Vanderah TW, Porreca F, Lai J (2005) Pain facilitatory cells in the rostral ventromedial medulla coexpress opioid-μ receptors and cholecystokinin type 2 receptors. Neuroscience 2005 Abstracts 394.17. Society for Neuroscience, Washington, DC

2004

Porecca F, Hruby V, Lai J (2003) CCK-SAP in Binding Studies. Targeting Trends 4(4).

2003

Treece BR, Speth RC, Ritter RC, Burns GA (2003) Altered CCK binding in the dorsal vagal complex following cytotoxic lesion of the nodose ganglion. Neuroscience 2003 Abstracts 830.5. Society for Neuroscience, New Orleans, LA

Xie Y, Vanderah TW, Ossipov MH, Lai J, Porreca F (2003) A single rostral ventromedial medulla (RVM) treatment with cholecystokinin-saporin (CCK-sap) prevents the development of opioid-induced paradoxical pain and spinal morphine antinociceptive tolerance. Neuroscience 2003 Abstracts 177.4. Society for Neuroscience, New Orleans, LA

Zhang J, Speth RC, Simasko S, Ritter RC (2003) Hypothalamic injection of targeted toxin for cholecystokinin receptive neurons leads to increased 24 hour food intake and weight gain. Neuroscience 2003 Abstracts 830.3. Society for Neuroscience, New Orleans, LA