Anti-SERT-SAP References

Cat #IT-23


Bernabe CS, Caliman IF, Truitt WA, Molosh AI, Lowry CA, Hay-Schmidt A, Shekhar A, Johnson PL. Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors. (2020) J Psychopharmacol 34(4):400-411. doi: 10.1177/0269881119900981

Objective:  To investigate the role of amygdala-projecting 5-HT neurons in the DR in innate anxiety and conditioned fear behaviors.
Summary:  The studies support the hypothesis that amygdala-projecting 5-HT neurons in the DR represent an anxiety and fear-on network.
Dose: Each rat received two bilateral microinjections per site (100 nL each, 1 μM in ACSF) of either Anti-SERT-SAP or the control Mouse IgG-SAP.


Bernabe C, Caliman I, de Abreu A, Dustrude E, Molosh A, Shekhar A, & Johnson P. S38. Dissecting the Functional Heterogeneity of Serotonergic Systems That Regulate Fear and Panic. (2019) Biol Psychiatry, 85 (10):S311. doi: 10.1016/j.biopsych.2019.03.789

Objective:  To elucidate the role of these serotonergic networks on learned fear and innate panic responses.
Summary: LED excitation or lesioning of PeF projecting 5-HT system respectively attenuated and enhanced panic-associated escape/flight behaviors and cardioexcitation following a 7.5 and 20% CO2 challenge.
Dose:  Anti-SERT-SAP was injected  into the BLA or PeF to lesion these 5-HT pathways.

Pawluski JL, Li M, Lonstein JS. Serotonin and motherhood: From molecules to mood. (2019) Front Neuroendocrinol 53:100742. doi: 10.1016/j.yfrne.2019.03.001

Summary:  Serotonin may affect how mothers perceive or behaviorally readjust to changes in the sensory cues emitted by their offspring as they age. The DR serotonin-lesioned mothers studied by Holschbach and colleagues (2018) were much less maternally aggressive, which was concomitant with reduced serotonin-immunoreactive fiber density in the anterior hypothalamus, a brain site previously implicated in serotonin’s influence on aggressive behaviors in male animals.


Holschbach MA, Vitale EM, Lonstein JS. Serotonin-specific lesions of the dorsal raphe disrupt maternal aggression and caregiving in postpartum rats. (2018) Behav Brain Res 348:53-64. doi: 10.1016/j.bbr.2018.04.008

KIT-23:  Anti-SERT-SAP, Mouse IgG-SAP
Objective:  To determine the effects of behavioral modifications associated with early motherhood by permanently disrupting serotonin signaling at one of its primary sources, the dorsal raphe (DR).
Summary:  Prepartum serotonin-specific lesions of the DRdm impaired maternal aggression.  Larger postpartum DR serotonin lesions affected both aggression and caregiving.  DR serotonin lesions did not affect postpartum anxiety,
Dose:  1 μL of 0.1M anti-SERT-SAP or control Mouse IgG-SAP was slowly infused into the DR.


Bernabe CS, Caliman IF, Abreu ARR, Shekhar A, Johnson PL (2016) A unique subdivision of serotonergic neurons in the dorsal raphe nucleus projects to the basolateral amygdala complex to enhance fear-conditioned behaviors. Soc Neurosci Meeting Abstract 74.23

Vitale EM, Holschbach MA, Lonstein JS (2016) Maternal aggression is impaired by prepartum serotonin-specific lesions of the midbrain dorsal raphe. Soc Neurosci Meeting Abstract 338.01


Nattie E, Li A (2009) Central chemoreception is a complex system function that involves multiple brain stem sites. J Appl Physiol 106:1464-1466. (Targeting Trends 09q3)


See also: Society for Neuroscience 2008 Abstracts


Dias MB, Nucci TB, Margatho LO, Antunes-Rodrigues J, Gargaglioni LH, Branco LG (2007) Raphe Magnus Nucleus is involved in ventilatory but not hypothermic response to CO2. J Appl Physiol 103(5):1780-1788. (Targeting Trends 07q4)

Gravitt K, Marson L (2007) Effect of the destruction of cells containing the serotonin reuptake transporter on urethrogenital reflexes. J Sex Med 4:322-330; discussion 330-321. (Targeting Trends 07q3)

Shen FM, Wang J, Ni CR, Yu JG, Wang WZ, Su DF (2007) Ketanserin-induced baroreflex enhancement in spontaneously hypertensive rats depends on central 5-HT(2A) receptors. Clin Exp Pharmacol Physiol 34:702-707. (Targeting Trends 07q4)


Wiley RG. (2006) Targeted Toxins in Pain. Targeting Trends 7(2).


See: Society for Neuroscience 2005 Abstracts


Nattie EE, Li A, Richerson GB, Lappi DA (2004) Medullary serotonergic neurons and adjacent neurons that express neurokinin-1 receptors are both involved in chemoreception in vivo. J Physiol 556(1):235-253. (Targeting Trends 04q2)

See also: Society for Neuroscience 2004 Abstracts


Feldman JL, Mitchell GS, Nattie EE (2003) Breathing: Rhythmicity, Plasticity, Chemosensitivity. Annu Rev Neurosci 26:239-266. (Targeting Trends 03q3)

See also: Society for Neuroscience 2003 Abstracts


See also: Forum of European Neuroscience 2002 Abstracts and Society for Neuroscience 2002 Abstracts