Anti-SERT-SAP References

Cat #IT-23


Bernabe CS, Caliman IF, Truitt WA, Molosh AI, Lowry CA, Hay-Schmidt A, Shekhar A, Johnson PL. Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors. (2020) J Psychopharmacol 34(4):400-411. doi: 10.1177/0269881119900981

Objective:  To investigate the role of amygdala-projecting 5-HT neurons in the DR in innate anxiety and conditioned fear behaviors.
Summary:  The studies support the hypothesis that amygdala-projecting 5-HT neurons in the DR represent an anxiety and fear-on network.
Dose: Each rat received two bilateral microinjections per site (100 nL each, 1 μM in ACSF) of either Anti-SERT-SAP or the control Mouse IgG-SAP.


Bernabe C, Caliman I, de Abreu A, Dustrude E, Molosh A, Shekhar A, & Johnson P. S38. Dissecting the Functional Heterogeneity of Serotonergic Systems That Regulate Fear and Panic. (2019) Biol Psychiatry, 85 (10):S311. doi: 10.1016/j.biopsych.2019.03.789

Objective:  To elucidate the role of these serotonergic networks on learned fear and innate panic responses.
Summary: LED excitation or lesioning of PeF projecting 5-HT system respectively attenuated and enhanced panic-associated escape/flight behaviors and cardioexcitation following a 7.5 and 20% CO2 challenge.
Dose:  Anti-SERT-SAP was injected  into the BLA or PeF to lesion these 5-HT pathways.

Pawluski JL, Li M, Lonstein JS. Serotonin and motherhood: From molecules to mood. (2019) Front Neuroendocrinol 53:100742. doi: 10.1016/j.yfrne.2019.03.001

Summary:  Serotonin may affect how mothers perceive or behaviorally readjust to changes in the sensory cues emitted by their offspring as they age. The DR serotonin-lesioned mothers studied by Holschbach and colleagues (2018) were much less maternally aggressive, which was concomitant with reduced serotonin-immunoreactive fiber density in the anterior hypothalamus, a brain site previously implicated in serotonin’s influence on aggressive behaviors in male animals.


Holschbach MA, Vitale EM, Lonstein JS. Serotonin-specific lesions of the dorsal raphe disrupt maternal aggression and caregiving in postpartum rats. (2018) Behav Brain Res 348:53-64. doi: 10.1016/j.bbr.2018.04.008

IT-23:  Anti-SERT-SAP, Mouse IgG-SAP
Objective:  To determine the effects of behavioral modifications associated with early motherhood by permanently disrupting serotonin signaling at one of its primary sources, the dorsal raphe (DR).
Summary:  Prepartum serotonin-specific lesions of the DRdm impaired maternal aggression.  Larger postpartum DR serotonin lesions affected both aggression and caregiving.  DR serotonin lesions did not affect postpartum anxiety,
Dose:  1 μL of 0.1M anti-SERT-SAP or control Mouse IgG-SAP was slowly infused into the DR.

Oliveira LM, Moreira TS, Takakura AC. Raphe Pallidus is Not Important to Central Chemoreception in a Rat Model of Parkinson’s Disease. (2018) Neuroscience 369:350-362. doi: 10.1016/j.neuroscience.2017.11.038

Objective: To investigate if serotonin-expressing neurons in the Raphe pallidus/parapyramidal region (RPa/PPy) are also involved in the modulation of breathing during central chemoreception activation in a PD animal model.


Bernabe CS, Caliman IF, Abreu ARR, Shekhar A, Johnson PL (2016) A unique subdivision of serotonergic neurons in the dorsal raphe nucleus projects to the basolateral amygdala complex to enhance fear-conditioned behaviors. Soc Neurosci Meeting Abstract 74.23

Vitale EM, Holschbach MA, Lonstein JS (2016) Maternal aggression is impaired by prepartum serotonin-specific lesions of the midbrain dorsal raphe. Soc Neurosci Meeting Abstract 338.01


Nattie E, Li A (2009) Central chemoreception is a complex system function that involves multiple brain stem sites. J Appl Physiol 106:1464-1466. (Targeting Trends 09q3)


Dias MB, Nucci TB, Margatho LO, Antunes-Rodrigues J, Gargaglioni LH, Branco LG (2007) Raphe Magnus Nucleus is involved in ventilatory but not hypothermic response to CO2. J Appl Physiol 103(5):1780-1788. (Targeting Trends 07q4)

Gravitt K, Marson L (2007) Effect of the destruction of cells containing the serotonin reuptake transporter on urethrogenital reflexes. J Sex Med 4:322-330; discussion 330-321. (Targeting Trends 07q3)

Shen FM, Wang J, Ni CR, Yu JG, Wang WZ, Su DF (2007) Ketanserin-induced baroreflex enhancement in spontaneously hypertensive rats depends on central 5-HT(2A) receptors. Clin Exp Pharmacol Physiol 34:702-707. (Targeting Trends 07q4)


Wiley RG. (2006) Targeted Toxins in Pain. Targeting Trends 7(2).


Nattie EE, Li A, Richerson GB, Lappi DA (2004) Medullary serotonergic neurons and adjacent neurons that express neurokinin-1 receptors are both involved in chemoreception in vivo. J Physiol 556(1):235-253. (Targeting Trends 04q2)


Feldman JL, Mitchell GS, Nattie EE (2003) Breathing: Rhythmicity, Plasticity, Chemosensitivity. Annu Rev Neurosci 26:239-266. (Targeting Trends 03q3)