CTB-SAP References

Cat #IT-14

2018

Nichols NL, Craig TA, & Tanner MA. Phrenic long-term facilitation following intrapleural CTB-SAP-induced respiratory motor neuron death. (2018). Respiratory Physiology & Neurobiology, 256 43-49.

Objective:  To study the impact of respiratory motor neuron death.
Summary:  Intrapleural CTB-SAP mimics aspects of ALS.  Seven days of CTB-SAP enhances respiratory plasticity.
Dose:  Bilateral intrapleural injections of:
1) CTB-SAP (25 μg), or 2) un-conjugated CTB and SAP (control).

Qiu M, Li J, Tan L, Zhang M, Zhou G, Zeng T, & Li A. Targeted Ablation of Distal Cerebrospinal Fluid-Contacting Nucleus Alleviates Renal Fibrosis in Chronic Kidney Disease. (2018). Front Physiol, 9 (1640).

Objective:  To test the hypothesis  that distal cerebrospinal fluid-contacting nucleus (dCSF-CNs) might affect the renin-angiotensin system (RAS) in kidney injury progression.
Summary:  Less CTB-labeled neurons were found in dCSF-CNs of CTB-SAP-treated rats. Meanwhile, CTB-SAP downregulated AGT, Ang II, AT1R, NOX2, catalase, MCP-1, IL-6, fibronectin, and collagen I, and upregulated ACE2 and Mas receptor. Targeted dCSF-CNs ablation could alleviate renal inflammation and fibrosis in chronic kidney injury.
Dose: CTB-SAP (500 ng) into the lateral ventricles  over a 3-min period.

Xiong L, Liu Y, Zhou M, Wang G, Quan D, Shuai W, Shen C, Kong B, Huang C, & Huang H. Targeted Ablation of Cardiac Sympathetic Neurons Attenuates Adverse Post-Infarction Remodeling and Left Ventricle Dysfunction. (2018). Exper Physiol, /doi.org/10.1113/EP086928.

Objective: To determine whether targeted ablation of cardiac sympathetic neurons (TACSN) could suppress myocardial infarction-induced adverse cardiac remodeling and left ventricle dysfunction.
Summary:  TACSN significantly alleviated sympathetic remodeling and neuroendocrine activation, attenuated cardiac hypertrophy and fibrosis, and improved the left ventricular function.  Thus, TACSN may have a beneficial effect on adverse post-infarction remodeling and left ventricle dysfunction.
Dose: 20 μl of CTB-SAP (1.2 mg/ml) was mixed with 4 μl of 3% Evans blue dye to make it visible (CTB-SAP is colorless), ensuring localization within the ganglia. The CTB-SAP/ Evan blue dye solution was slowly and intermittently injected into the left stellate ganglia using a glass micropipette.

Xiong L, Liu Y, Zhou M, Wang G, Quan D, Shen C, Shuai W, Kong B, Huang C, & Huang H. Targeted Ablation of Cardiac Sympathetic Neurons Improves Ventricular Electrical Remodelling in a Canine Model of Chronic Myocardial Infarction. (2018). EP Europaceeuy090-euy090.

Objective: To evaluate the cardiac electrophysiologic effects of targeted ablation of cardiac sympathetic neurons (TACSN) in a canine model of chronic myocardial infarction (MI).
Summary: Targeted ablation of cardiac sympathetic neuron attenuates sympathetic remodelling and improves ventricular electrical remodelling in the chronic phase of MI. These data suggest that TACSN may be a novel approach to treating ventricular arrhythmias.
Dose: 20 μl of CTB-SAP (1.2 mg/ml) was mixed with 4 μl of 3% Evans blue dye to make it visible (CTB-SAP is colorless), ensuring localization within the ganglia. The CTB-SAP/ Evan blue dye solution was slowly and intermittently injected into the left stellate ganglia using a glass micropipette.

Song S-Y, & Zhang L-C. (2018). The Establishment of a CSF-Contacting Nucleus “Knockout” Model Animal. Frontiers Neuroanat (Vol. 12).
DOI:10.3389/fnana.2018.00022

Objective:  To establish a cerebrospinal fluid (CSF)-contacting nucleus-deficient model animal using cholera toxin B subunit-saporin (CTB-SAP).
Summary:  The complete ablation occurred by Day 7 after CTB-SAP microinjection. A model animal that had no CSF-contacting nucleus was established after survival beyond that time point.  No obvious effects were observed in the vital status of the model animals, and their survival was ensured.  The common physiological parameters of model animals were stable.  The present study provides a method to establish a CSF-contacting nucleus “knockout”  model animal, which is similar to a gene knockout model animal for studying this particular nucleus in vivo.
Dose:  3 μl (500 ng) CTB-SAP was microinjected into the lateral ventricle.

2017

Cai Y, Chew C, Muñoz F, Sengelaub DR. (2017) Neuroprotective Effects of Testosterone Metabolites and Dependency on Receptor Action on the Morphology of Somatic Motoneurons Following the Death of Neighboring Motoneurons. Dev Neurobiol 77(6):691-707. PMID: 27569375 (Targeting Trends 17q1)

Gulino R, Parenti R, Gulisano M. (2017) Sonic Hedgehog and Tdp-43 Participate in the Spontaneous Locomotor Recovery in a Mouse Model of Spinal Motoneuron Disease. J Funct Morphol Kinesiol 2:11. (read summary)

2016

Fei Y, Wang X, Chen S, Zhou Q, Zhang C, Li Y, Sun L, Zhang L. (2016) Role of the RVM in Descending Pain Regulation Originating from the Cerebrospinal Fluid-Contacting Nucleus. Neurochem Res 41(7):1651-1661. PMID: 26961890 (Targeting Trends 16q3)

Gulino R. (2016) Neuroplasticity and Repair in Rodent Neurotoxic Models of Spinal Motoneuron Disease. Neural Plast 2016:2769735. PMID: 26862439 (Targeting Trends 16q2)

Gulino R, Forte S, Parenti R, Gulisano M (2016) Novel targets for modulation of plasticity in a mouse model of motoneuron degeneration. Soc Neurosci Meeting Abstract 812.14

Xia W, Liu Y. (2016) Targeted Ablation of Cardiac Sympathetic Neurons: A Promising Approach to Prevent Sudden Cardiac Death. Int J Cardiol 202:425-26. PMID: 26433164 (Targeting Trends 17q1)

2015

Gulino R, Parenti R, Gulisano M. (2015) Novel Mechanisms of Spinal Cord Plasticity in a Mouse Model of Motoneuron Disease. Biomed Res Int 2015:654637. (Targeting Trends 15q3)

Nichols NL, Vinit S, Bauernschmidt L, Mitchell GS. (2015) Respiratory function after selective respiratory motor neuron death from intrapleural CTB-saporin injections. Exp Neurol 267:18-29 (Targeting Trends 15q1)

Xing D, Wu Y, Li G, Song S, Liu Y, Liu H, Wang X, Fei Y, Zhang C, Li Y, Zhang L. (2015) Role of cerebrospinal fluid-contacting nucleus in sodium sensing and sodium appetite. Physiol Behav 147:291-299. (Targeting Trends 15q3)

Wu YH, Song SY, Liu H, Xing D, Wang X, Fei Y, Li GL, Zhang C, Li Y, Zhang LC. (2015) Role of adrenomedullin in the cerebrospinal fluid-contacting nucleus in the modulation of immobilization stress. Neuropeptides 51:43-54. (Targeting Trends 15q3)

2014

Cao J WT, Zhang LC. (2014) Targeted damage of the cerebrospinal fluid-contacting nucleus contributes to the pain behavior and the expression of 5-HT and c-Fos in the spinal dorsal horn of rats. Zhongguo Ying Yong Sheng Li Xue Za Zhi 30(3):218-222. (Targeting Trends 14q4)

Liu H, Yan WW, Lu XX, Zhang XL, Wei JQ, Wang XY, Wang T, Wu T, Cao J, Shao CJ, Zhou F, Zhang HX, Zhang P, Zang T, Lu XF, Cao JL, Ding HL, Zhang LC. (2014) Role of the cerebrospinal fluid-contacting nucleus in the descending inhibition of spinal pain transmission. Exp Neurol 261C:475-485. (Targeting Trends 14q4)

2013

Gulino R, Gulisano M. (2013) Noggin and Sonic hedgehog are involved in compensatory changes within the motoneuron-depleted mouse spinal cord. J Neurol Sci 332(1-2):102-109. (Targeting Trends 13q4)

2012

Gulino R, Gulisano M. (2012) Involvement of brain-derived neurotrophic factor and sonic hedgehog in the spinal cord plasticity after neurotoxic partial removal of lumbar motoneurons. Neurosci Res 73(3):238-247. (Targeting Trends 12q3)

2011

See also: International Brain Research Organization 2011 Abstracts

2010

Cerminara NL (2010) Cerebellar modules: individual or composite entities? J Neurosci 30(48):16065-16067. (Targeting Trends 11q1)

Gulino R, Perciavalle V, Gulisano M (2010) Expression of cell fate determinants and plastic changes after neurotoxic lesion of adult mice spinal cord by cholera toxin-B saporin. Eur J Neurosci 31(8):1423-1434. (Targeting Trends 10q3)

Gulino R, Perciavalle V, Gulisan M. (2010) Role of Cell Fate Determinants in a Model of Spinal Cord Neurotoxic Lesion Induced by Cholera Toxin B-Saporin. Targeting Trends 11(3).

Lujan HL, DiCarlo SE. (2010) Targeted Ablation of Sympathetic Neurons Reduces Ventricular Arrhythmias and Autonomic Dysreflexia. Targeting Trends 11(4).

Lujan HL, Palani G, Peduzzi J, Dicarlo SE (2010) Targeted Ablation of Mesenteric Projecting Sympathetic Neurons Reduces the Hemodynamic Response to Pain in Conscious Spinal Cord Transected Rats. Am J Physiol Regul Integr Comp Physiol 298(5):R1358-1365. (Targeting Trends 10q3)

Lujan HL, Palani G, Zhang L, Dicarlo SE (2010) Targeted Ablation of Cardiac Sympathetic Neurons Reduces the Susceptibility to Ischemia-Induced Sustained Ventricular Tachycardia in Conscious Rats. Am J Physiol Heart Circ Physiol 298:H1330-H1339. (Targeting Trends 10q2)

See also: Society for Neuroscience 2010 Abstracts

2009

Fargo KN, Foster AM, Sengelaub DR (2009) Neuroprotective effect of testosterone treatment on motoneuron recruitment following the death of nearby motoneurons. Dev Neurobiol 69:825-835. (Targeting Trends 09q4)

Little CM, Coons KD, Sengelaub DR (2009) Neuroprotective effects of testosterone on the morphology and function of somatic motoneurons following the death of neighboring motoneurons. J Comp Neurol 512:359-372. (Targeting Trends 09q2)

Lujan HL, Palani G, Chen Y, Peduzzi JD, Dicarlo SE (2009) Targeted ablation of cardiac sympathetic neurons reduces resting, reflex and exercise-induced sympathetic activation in conscious rats. Am J Physiol Heart Circ Physiol 296:H1305-1311. (Targeting Trends 09q3)

Wilson RE, Coons KD, Sengelaub DR (2009) Neuroprotective effects of testosterone on dendritic morphology following partial motoneuron depletion: efficacy in female rats. Neurosci Lett 465:123-127. (Targeting Trends 10q1)

See also: Society for Neuroscience 2009 Abstracts

2008

Pijpers A, Winkelman BH, Bronsing R, Ruigrok TJ (2008) Selective impairment of the cerebellar C1 module involved in rat hind limb control reduces step-dependent modulation of cutaneous reflexes. J Neurosci 28:2179-2189. (Targeting Trends 08q2)

See also: Society for Neuroscience 2008 Abstracts

2007

See: Society for Neuroscience 2007 Abstracts

2006

Wu M, Kc P, Mack SO, Haxhiu MA (2006) Ablation of vagal preganglionic neurons innervating the extra-thoracic trachea affects ventilatory responses to hypercapnia and hypoxia. Respir Physiol Neurobiol 152(1):36-50. (Targeting Trends 05q4)

See also: Society for Neuroscience 2006 Abstracts

2005

Lappi DA (2005) Targeted Toxins from Here to There. Targeting Trends 6(3):1, 6.

See: Society for Neuroscience 2005 Abstracts

2004

Fargo KN, Sengelaub DR (2004) Testosterone manipulation protects motoneurons from dendritic atrophy after contralateral motoneuron depletion. J Comp Neurol 469(1):96-106. (Targeting Trends 04q2)

Fargo KN, Sengelaub DR (2004) Exogenous testosterone prevents motoneuron atrophy induced by contralateral motoneuron depletion. J Neurobiol 60(3):348-359. (Targeting Trends 05q1)(Targeting Trends 05q1)

Jasmin L, Ohara PT (2004) Recurrent paraplegia after remyelination of the spinal cord. J Neurosci Res 77(2):277-284. (Targeting Trends 04q4)(Targeting Trends 04q4)

See also: Society for Neuroscience 2004 Abstracts

2003

Farhadi HF, Lepage P, Forghani R, Friedman HC, Orfali W, Jasmin L, Miller W, Hudson TJ, Peterson AC (2003) A combinatorial network of evolutionarily conserved myelin basic protein regulatory sequences confers distinct glial-specific phenotypes. J Neurosci 23(32):10214-10223. (Targeting Trends 04q1)

See: Society for Neuroscience 2003 Abstracts

2002

See: Society for Neuroscience 2002 Abstracts

2001

See: Society for Neuroscience 2001 Abstracts

2000

Jasmin L, Janni G, Moallem TM, Lappi DA, Ohara PT (2000) Schwann cells are removed from the spinal cord after effecting recovery from paraplegia. J Neurosci 20(24):9215-9223.