Meng J, Ni J, Wu Z, Jiang M, Zhu A, Qing H, & Nakanishi H. The Critical Role of Il-10 in the Antineuroinflammatory and Antioxidative Effects of Rheum Tanguticum on Activated Microglia. (2018). Oxid Med Cell Longev, 2018 (Article ID 1083596):12.
Objective: To investigate anti-inflammatory and antioxidative effects of a traditional Tibetan medicine (Rheum tanguticum; RT) on activated microglia.
Summary: RT may be useful for the pharmacological intervention against excessive inflammatory and oxidative responses associated with AD by inducing the production of IL-10 by microglia.
Dose: Mac-1-SAP (1.3nM) was applied to hippocampal slice cultures 24 h prior to stimulation with chromogranin A or pancreastatin.
Acharjee S, Verbeek M, Gomez CD, Bisht K, Lee B, Benoit L, Sharkey KA, Benediktsson A, Tremblay M-E, & Pittman QJ. Reduced Microglial Activity and Enhanced Glutamate Transmission in the Basolateral Amygdala in Early CNS Autoimmunity. (2018). The J Neurosci, 38 (42):9019.
Cat. #IT-06 Objective: To identify CNS changes associated with behaviors in multiple sclerosis (MS) patients.
Summary: The data from this study reveal increased synaptic activity and spine density in early stages of experimental autoimmune encephalomyelitis (an animal model of MS) in the basolateral amygdala.
Dose: Mac-1-SAP mouse/human or Rat-IgG-SAP (control) was injected unilaterally in the BLA (1mcg/1mcl).
Mousseau M, Burma NE, Lee KY, Leduc-Pessah H, Kwok CHT, Reid AR, O’Brien M, Sagalajev B, Stratton JA, Patrick N, Stemkowski PL, Biernaskie J, Zamponi GW, Salo P, McDougall JJ, Prescott SA, Matyas JR, & Trang T. Microglial pannexin-1 channel activation is a spinal determinant of joint pain. (2018). Science Advances, 4 (8):PMID: 30101191
Objective: To identify therapeutic targets for alleviating mechnical allodynia, a sign/symptom of arthritis.
Summary: The pannexin-1 (Panx1) channel is validated as a target; blockade of P2X7 receptors or ablation of spinal microglia prevented and reversed mechanical allodynia.
Dose: Mac-1-SAP and unconjugated Saporin (15 mg per intrathecal injection on days 0, 1, and 2). The specific depletion of spinal lumbar microglia attenuated the development of MIA-induced hypersensitivity indicating that spinal microglia causally contribute to the development of mechanical allodynia. By contrast, intrathecal injection of Control (unconjugated Saporin) did not alter the development of MIA-induced mechanical allodynia.
SeungHwan L, Xiang Qun S, Anni F, Brian W, & Ji Z. Targeting Macrophage and Microglia Activation with Colony Stimulating Factor 1 Receptor Inhibitor Is an Effective Strategy to Treat Injury-Triggered Neuropathic Pain. (2018). Molecular Pain, 14 1744806918764979.
Depletion of spinal microglia with selectively immunotoxin, Mac-1-SAP was able to prevent and reverse neuropathic pain behavior.
Cites: Echeverry S, Shi XQ, Yang M, Huang H, Wu Y, Lorenzo LE, Perez-Sanchez J, Bonin RP, De Koninck Y and Zhang J. Spinal microglia are required for long-term maintenance of neuropathic pain. Pain 2017; 158: 1792–1801.)
Echeverry S, Shi XQ, Yang M, Huang H, Wu Y, Lorenzo LE, Perez-Sanchez J, Bonin RP, De Koninck Y, & Zhang J. Spinal Microglia Are Required for Long-Term Maintenance of Neuropathic Pain. (2017). Pain, 158 (9):1792-1801. 2017/07/27.
Selective depletion of spinal microglia in male rats with the targeted immunotoxin Mac-1-SAP and blockade of brain derived neurotrophic factor–TrkB signalling with intrathecal TrkB Fc chimera, but not cytokine inhibition, almost completely reversed pain hypersensitivity. To selectively deplete microglia in the spinal cord, Mac-1-SAP was injected intrathecally. In each group, rats received either an intrathecal injection of 12 mg/7 mL of Mac-1-SAP (n = 6-8) or equal volume of 0.9% saline (n 5 6) or the inactive unconjugated toxin, Saporin (n = 6).)
Leduc-Pessah H, Weilinger NL, Fan CY, Burma NE, Thompson RJ, Trang T. (2017) Site-Specific Regulation of P2x7 Receptor Function in Microglia Gates Morphine Analgesic Tolerance. J Neurosci 37(42):10154-72. PMID: 28924009
Rosen SF, Ham B, Drouin S, Boachie N, Chabot-Dore AJ, Austin JS, Diatchenko L, Mogil JS. (2017) T-Cell Mediation of Pregnancy Analgesia Affecting Chronic Pain in Mice. J Neurosci 37(41):9819-27. PMID: 28877966
Yao Y, Echeverry S, Shi XQ, Yang M, Yang QZ, Wang GY, Chambon J, Wu YC, Fu KY, De Koninck Y, Zhang J. (2016) Dynamics of spinal microglia repopulation following an acute depletion. Sci Rep 6:22839. PMID: 26961247 (Targeting Trends 16q3)
Li J, Pan Y, Zhang B, Chen Q. (2015) Macrophages are needed in the progression of tuberculosis into lung cancer. Tumour Biol 36(8):6063-6066. (Targeting Trends 15q2)
Sorge RE, Mapplebeck JC, Rosen S, Beggs S, Taves S, Alexander JK, Martin LJ, Austin JS, Sotocinal SG, Chen D, Yang M, Shi XQ, Huang H, Pillon NJ, Bilan PJ, Tu Y, Klip A, Ji RR, Zhang J, Salter MW, Mogil JS. (2015) Different Immune Cells Mediate Mechanical Pain Hypersensitivity in Male and Female Mice. Nat Neurosci 18(8):1081-83. PMID: 26120961 (Targeting Trends 17q1)
Wang B, Miao Y, Zhao Z, Zhong Y. (2015) Inflammatory Macrophages Promotes Development of Diabetic Encephalopathy. Cell Physiol Biochem 36(3):1142-1150. (Targeting Trends 15q3)
Criscimanna A, Coudriet GM, Gittes GK, Piganelli JD, Esni F. (2014) Activated Macrophages Create Lineage-specific Microenvironments for Pancreatic Acinar- and beta-cell Regeneration in Mice. Gastroenterology 147(5):1106-1118.e11. (Targeting Trends 14q4)
Nunan R, Sivasathiaseelan H, Khan D, Zaben M, Gray W. (2014) Microglial VPAC1R mediates a novel mechanism of neuroimmune-modulation of hippocampal precursor cells via IL-4 release. Glia 62(8):1313-1327. (Targeting Trends 14q4)
Ferrini F, Koninck YD. (2013) Role of spinal microglia in the development of morphine-induced hyperalgesia. Targeting Trends 14(4).
Ferrini F, Trang T, Mattioli TA, Laffray S, Del’Guidice T, Lorenzo LE, Castonguay A, Doyon N, Zhang W, Godin AG, Mohr D, Beggs S, Vandal K, Beaulieu JM, Cahill CM, Salter MW, De Koninck Y. (2013) Morphine hyperalgesia gated through microglia-mediated disruption of neuronal Cl(-) homeostasis. Nat Neurosci 16(2):183-192. (Targeting Trends 13q2)
Heldmann U, Mine Y, Kokaia Z, Ekdahl CT, Lindvall O. (2011) Selective depletion of Mac-1-expressing microglia in rat subventricular zone does not alter neurogenic response early after stroke. Exp Neurol 229(2):391-398. (Targeting Trends 11q2)
See also: Society for Neuroscience 2010 Abstracts
Montero M, Gonzalez B, Zimmer J. (2009) Depletion of Microglia by Mac-1-SAP in Mouse Hippocampal Slice Cultures Enhances Ischemia-Like Neurodegeneration. Targeting Trends 10(3).
Montero M, Gonzalez B, Zimmer J (2009) Depletion of Microglia by Mac-1-SAP in Mouse Hippocampal Slice Cultures Enhances Ischemia-Like Neurodegeneration. Targeting Trends 10(3):1, 6.
Montero M, Gonzalez B, Zimmer J (2009) Immunotoxic depletion of microglia in mouse hippocampal slice cultures enhances ischemia-like neurodegeneration. Brain Res 1291:140-152. (Targeting Trends 09q4)
Domico LM, Cooper KR, Bernard LP, Zeevalk GD (2007) Reactive oxygen species generation by the ethylene-bis-dithiocarbamate (EBDC) fungicide mancozeb and its contribution to neuronal toxicity in mesencephalic cells. Neurotoxicology 28:1079-1091. (Targeting Trends 08q2)
Garcia-Alloza M, Ferrara BJ, Dodwell SA, Hickey GA, Hyman BT, Bacskai BJ (2007) A limited role for microglia in antibody mediated plaque clearance in APP mice. Neurobiol Dis 28(3):286-292. (Targeting Trends 07q4)
Zhao P, Waxman SG, Hains BC (2007) Extracellular signal-regulated kinase-regulated microglia-neuron signaling by prostaglandin E2 contributes to pain after spinal cord injury. J Neurosci 27:2357-2368. (Targeting Trends 07q2)
See also: Society for Neuroscience 2007 Abstracts
Kanai T, Uraushihara K, Totsuka T, Nemoto Y, Fujii R, Kawamura T, Makita S, Sawada D, Yagita H, Okumura K, Watanabe M. (2006) Ameliorating effect of saporin-conjugated anti-CD11b monoclonal antibody in a murine T-cell-mediated chronic colitis. J Gastroenterol Hepatol 21(7):1136-1142. (Targeting Trends 06q4)
Dommergues MA, Plaisant F, Verney C, Gressens P (2003) Early microglial activation following neonatal excitotoxic brain damage in mice: a potential target for neuroprotection. Neuroscience 121(3):619-628. (Targeting Trends 04q1)
Kanai T, Uraushihara K, Okazawa A, Hibi T, Watanabe M (2003) Macrophage-derived IL-18 targeting for the treatment of Crohn’s disease. Curr Drug Targets Inflamm Allergy 2(2):131-136. (Targeting Trends 04q1)
Kanai T, Uraushihara K, Totsuka T, Nemoto Y, Fujii R, Kawamura T, Makita S, Sawada D, Yagita H, Okumura K, Watanabe M (2006) Ameliorating effect of saporin-conjugated anti-CD11b monoclonal antibody in a murine T-cell-mediated chronic colitis. J Gastroenterol Hepatol 21(7):1136-1142. (Targeting Trends 06q4)
Kanai T, Watanabe M, Okazawa A, Sato T, Yamazaki M, Okamoto S, Ishii H, Totsuka T, Iiyama R, Okamoto R, Ikeda M, Kurimoto M, Takeda K, Akira S, and Hibi T (2001) Macrophage-derived IL-18-mediated intestinal inflammation in the murine model of Crohn’s disease. Gastroenterol 121:875-888. (Targeting Trends 02q1)