Retinol binding protein 4(RBP4) belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli.
RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the rbp-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
RBP-4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids and having a molecular mass of 23 kDa. RBP4 is fused to N-terminus His tag which contains additional 21 amino acids. RBP4 sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 amino acids 16-199. The Retinol Binding Protein-4 is purified by proprietary chromatographic techniques. RBP-4 His tag was filtered (0.4 um) and lyophilized from 0.5 mg/ml in PBS buffer, pH 7.5. Purity is greater than 95.0% as determined byRP-HPLC and SDS-PAGE. Biological Activity determined by its ability to bind all trans retinol acids. The binding of RBP4 to all trans retinol acids results in quenching of Trp fluroscence. 0.5M retinol acids/per M RBP4.
Amino acid sequence: MSWWHHHHHH NWNIPTTQDT TERDCRVSSF RVKENFDKAR FSGTWYAMAK KDPEGLFLQD NIVAEFSVDE TGQMSATAKG RVRLLNNWDV CADMVGTFTD TEDPAKFKMK YWGVASFLQK GNDDHWIVDT DYDTYAVQYS CRLLNLDGTC ADSYSFVFSR DPNGLPPEAQ KIVRQRQEEL CLARQYRLIV HNGYCDGRSERNLL.
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