Protein A is a cell-wall protein derived from Staphylococcus aureus which has unique binding properties to a variety of mammalian species of IgG. It can also bind some IgM and IgA. Protein A binds the Fc region of immunoglobulins through interaction with the heavy chain. It can be coupled to a variety of reporter molecules, such as fluorescent dyes, enzyme markers, biotin, colloidal gold, and radioactive iodine without affecting the antibody binding site. The recombinant version of protein A was developed to increase the specificity for IgG.
The recombinant protein A is produced by expressing a modified protein A gene in E. coli. It is a non-glycosylated, polypeptide chain having a molecular mass of 45 kDa. The protein solution contains no additives. Purity is greater than 98% as determined by RP-HPLC. Human IgG is not used in the purification of protein A. This protein A does not contain endotoxin that is frequently found in native protein A.
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