CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell will either have CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized.
Rat anti-mouse CD4 is purified by ion exchange column. It is conjugated to FITC. Protein concentration is 1 mg/ml in PBS (after reconstitution).
Reported to be effective for flow cytometry staining, immunohistochemistry, immunoblotting (western blot).
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