HTLV-1 is the etiological agent for adult T-cell leukemia, encoding the 40 kDa cell-transforming oncoprotein Tax. Tax protein sensitizes cells to apoptotic cell death induced by DNA damaging agents. Constitutive activation of TAK1 by HTLV-1 Tax-dependent overexpression of TAB2 induces activation of JNK-ATF2 but not IKK-NF-kB.
Anti-HTLV-1 TAX is purified by ion-exchange chromatography. The titer is determined by direct ELISA, a 1:50,000 dilution will yield an OD of 0.5 using an alkaline phosphatase secondary antibody.
Reported to be effective for ELISA, immunoblotting (western blot), immunoprecipitation.
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