IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Rat anti-mouse interleukin-10 (IL-10) is purified by boric acid precipitation. Protein concentration is 1 mg/ml in PBS (after reconstitution).
Reported to be effective for direct ELISA, western blot, immunoprecipitation, immunohistochemistry, intracellular staining.
To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols