Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. When first encountering an antigen, the primary cells to encounter it are the macrophages who phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as neutrophil chemotactic Factor.
Mouse anti-human interleukin-8 (IL-8) is purified over an ion exchange column. Protein concentration is 1 mg/ml in PBS (after reconstitution). This antibody was produced in BALB/c mice. No cross reactivity with murine IL-8 was detected.
Reported to be effective for direct ELISA, western blot, immunoprecipitation, and immunohistochemistry.
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