Targeted SAP conjugates are powerful and specific lesioning agents used in the technique known as Molecular Surgery. The ribosome-inactivating protein, saporin (from the seeds of the plant, Saponaria officinalis) is bound to a targeting agent (anything that is recognized on the cell surface and internalized). The targeted conjugate is administered to cells (in vitro or in vivo). The targeting agent seeks out and binds to its target on the cell surface. The conjugate is internalized, saporin breaks away from the targeting agent, and inactivates the ribosomes, which causes protein inhibition and, ultimately, cell death. Cells that do not have the cell surface marker are not affected.
Vesicular GABA transporters (vGAT) mediate the accumulation of GABA into synaptic vesicles and its release from nerve terminals. This transporter is expressed in the nerve endings of GABAergic neurons throughout the CNS and has also been found in the pancreas and pituitary gland. During development, expression of the vGAT protein changes. Expression can also change in response to patterns of neuronal activity.
Anti-vGAT-SAP specifically eliminates cells that express the vesicular GABA transporter.
To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols