Biotin-Z Antibody Internalization Kit (KIT-27-Z)

a tool to “piggyback” onto YOUR biotinylated material via streptavidin; targeting cells that recognize YOUR biotinylated material, eliminated via saporin
SKU: KIT-27-Z Category: Quantity: Kit (25ug) w/controls & developing reagents, Kit (100 ug) w/controls & developing reagents, Kit (250 ug) w/controls & developing reagents | Conjugate: saporin | Usage: converts biotinylated materials, eliminates cells |

Streptavidin-ZAP is a chemical conjugate of streptavidin and the ribosome-inactivating protein, saporin. It converts biotinylated materials into targeted toxins. This kit converts biotinylated materials into targeted toxins and will allow you to evaluate the ability of your reagent to internalize upon binding to its receptor. It is the perfect tool to screen your biotinylated antibody in vitro.

This kit includes Streptavidin-ZAP (IT-27) and BIgG-SAP control conjugate for your primary antibody host species and Saporin (PR-01) plus reagents for developing a cytotoxicity assay (PMS, XTT).

ATS recommends using a species-specific ZAP Antibody Internalization Kit when possible. View available kits here.

Other ZAP Conjugates:

Need another ZAP Conjugate for your target? Check here for other species & targets including in vivo and in vitro options.

keywords: Streptavidin, Avidin, Biotin, screening, ADC, Antibody Drug Conjugate, saporin, streptavidinylated, Streptavidin-SAP, Streptavidin-ZAP, Strep-ZAP, Strep-SAP, Biotin-Z, in vitro

Reviews

There are no reviews yet.

Be the first to review “Biotin-Z Antibody Internalization Kit (KIT-27-Z)”

View Data Sheet

Streptavidin-saporin: Converting biotinylated materials into targeted toxins

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2023) Streptavidin-saporin: Converting biotinylated materials into targeted toxins. Toxins 15(3):181. doi: 10.3390/toxins15030181

Summary: This manuscript describes the myriad of ways Streptavidin-ZAP is used and how this technology supports the scientific process of ‘Molecular Surgery’ and progress in research and drug development. Insights from publications and research performed using Streptavidin-ZAP and its impact on academia and industry for research and drug development are presented.

Read the full article.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom. Toxins (Basel) 14(3):184. doi: 10.3390/toxins14030184

Read complete article.

Antibody drug conjugates targeted to CD45 or CD117 enable allogeneic hematopoietic stem cell transplantation in animal models

Palchaudhuri R, Hyzy SL, Proctor JL, Adams HL, Pearse BR, Sarma G, Aslanian S, Gillard G, Lamothe TL, Burenkova O, Brooks ML, Gabros AD, McDonagh CF, Boitano AE, Cooke MP (2018) Antibody drug conjugates targeted to CD45 or CD117 enable allogeneic hematopoietic stem cell transplantation in animal models. Blood 132:3324. doi: 10.1182/blood-2018-99-119432

Objective: To further investigate and develop the utility of CD45-SAP and CD117-SAP, in combination with immunosuppression, in murine transplant models using i.v. administration in an allogeneic minor mismatch transplant model (Balb/c donor into DBA/2 recipients).

Summary: CD45-SAP or CD117-SAP in combination with immunosuppressants (30Fll and post-transplant Cytoxan) enabled >85% peripheral donor chimerism at 12 weeks post-transplantation. CD45-SAP and CD117-SAP were more effective at conditioning versus 2Gy TBI or pretransplant Cytoxan.

Usage: CD45-SAP (1.9 mg/kg, iv) and CD117-SAP (1mg/kg, iv) in an allogeneic minor mismatch transplant model (Balb/c donor into DBA/2 recipients).

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Antibody-based preparative regimens for cell, tissue and organ transplantation

Van Hentenryck M, Li Z, Murphy PM, Czechowicz A (2022) Antibody-based preparative regimens for cell, tissue and organ transplantation. (eds. 162). OBM Transplantation 6(3):162. doi: 10.21926/obm.transplant.2203162

Objective: Provide a review of progress in the use of antibodies to support cell and tissue transplantation with a particular focus on induction of donor-specific tolerance for solid organ transplantation.

Summary: Antibody-based conditioning to prepare the recipient is a promising approach towards achieving transplant tolerance in both hematopoietic and solid organ transplant settings.

Usage: To enhance HSC depletion while avoiding bystander toxicity (neutropenia, lymphopenia, and thrombocytopenia) caused by CD45-radioimmunotherapy, Palchaudhuri et al. developed a saporin-based CD45 (CD45-SAP) immunotoxin using a biotinylated antibody and Streptavidin-ZAP.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

See Also:

Evaluate Potential Targeting Molecules.

Kohls M (2006) Evaluate Potential Targeting Molecules. Nature Methods

Summary: Targeted toxins -- targeting agents conjugated to saporin -- are widely used to eliminate specific cell populations both in vitro and in vivo. For these molecules to be effective, it is vital that the targeting component of the conjugate specifically binds the cells of interest. A secondary conjugate, Streptavidin-ZAP, has been created by attaching the toxin saporin to streptavidin. The user can combine primary biotinylated material with Streptavidin-ZAP to quickly and economically screen potential targeting molecules for internalization and specificity. Once the appropriate targeting molecule is identified, a direct conjugation with saporin can be performed.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Read the article.

browse all references for this product | back to top

Streptavidin-ZAP (Cat. #IT-27)

Streptavidin-ZAP Antibody Kit, in vivo (KIT-27-A)

Streptavidin-ZAP Peptide Kit, in vivo (KIT-27-B)

Streptavidin-ZAP Kit, in vivo or in vitro (KIT-27-S)

Biotin-Z Antibody Internalization Kit, in vitro (KIT-27-Z)

Biotin-Z Peptide Internalization Kit, in vitro (KIT-27-ZB)

Biotin-Z Internalization Kit, in vitro (KIT-27-ZS)

FITC-Streptavidin-ZAP (Cat. #IT-85)

MonoBiotin-ZAP (Cat. #BT-ZAP)

Streptavidin-pHast (Cat. #PH-03)

MonoBiotin-ZAP (Cat. #BT-ZAP)

Streptavidin-ZAP Concentration 

Equimolar Mixing of Strep-ZAP and Biotinylated Molecule

DMSO% for peptide and Streptavidin-ZAP

Streptavidin-ZAP concentration ratio

Biotin-Z Internalization Kit Introduction

Biotin-Z Internalization Kit Tutorial

Biotin-Z (Streptavidin-ZAP) Kit Literature

Cytotoxicity Assay Protocol for ZAP Biotin-Z Internalization Kits

Online Calculator: Preparing ZAP Biotin-Z Kit Samples

Concentration Calculations: Convert molarity to mg/ml and mg/ml to molarity

Preparing and Interpreting Cytotoxicity Data in vitro


browse all protocols and calculators | back to top

Shopping Cart
Scroll to Top