Hum-ZAP uses your primary human monoclonal IgG antibody to target and eliminate cells that recognize your primary antibody. This secondary conjugate uses the secondary antibody (affinity-purified goat anti-human IgG) to “piggyback” onto YOUR human primary antibody. Hum-ZAP can be utilized for screening human IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
Hum-ZAP uses your human primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
Three sizes are available; each kit includes assay reagents and the following aliquots of Hum-ZAP and control:
KIT-22-Z (tests 1 ab) includes 25 mcg each of Hum-ZAP and Control Goat-IgG-SAP
KIT-22-Z4 (tests 4 abs) includes 100 mcg each of Hum-ZAP and Control Goat-IgG-SAP
KIT-22-Z10 (tests 10 abs) includes 250 mcg each of Hum-ZAP and Control Goat-IgG-SAP