Angiotensin II interacts with two types of G-protein coupled membrane receptors, AT-1 (type 1) and AT-2 (type 2). AT-2 is a seven transmembrane domain G-protein coupled membrane receptor comprised of 363 aa. It is highly expressed in myometrium, with lower levels in adrenal and fallopian tube in rats. It is also expressed at high levels in fetal kidney and intestine.
This antibody recognizes the Angiotensin II type 2 receptor (AT-2) in rat. The antisera was generated in rabbits by immunization with a synthetic peptide corresponding to a cytoplasmic region of the receptor conjugated to keyhole limpet hemocyanin (KLH). Antisera was then affinity-purified by passage through an affinity column cross-linked with the same peptide used for immunization. It has been conjugated to the fluorescent dye Alexa488.
Applications include immunolabeling and immunohistochemistry.
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