FITC-labeled Anti-DAT-ECD can be used to verify binding specificity of a targeted toxin to a cell line expressing the target molecule. By first binding the targeted toxin to fixed cells, then binding FITC-labeled Anti-DAT-ECD to the targeted toxin, specificity can be confirmed through the use of competing molecules or a control cell line. FITC is excited by 488 nm wavelength light, and emits at 525 nm.
This antibody recognizes the second extracellular loop of the dopamine transporter (DAT-ECD) in rat and human. It was produced in rat by immunization with a GST-DAT-loop fusion protein, then construction of a hybridoma with the murine nonsecreting myeloma cell line Sp2/0. The second extracellular loop, consisting of amino acids 180-218, was used to construct the fusion protein. The antibody was conjugated to FITC (5-iodoacetamidofluorescein) using SPDP.
Applications include flow cytometry.
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