Saporin Goat Polyclonal, FITC-labeled affinity-purified [FL-02]

SKU: FL-02 Categories: , , Antibody Type: affinity-purified, Polyclonal | Host: Goat | Conjugate: FITC | Usage: flow cytometry |

FITC-labeled Anti-SAP can be used to verify binding specificity of a targeted toxin to a cell line expressing the target molecule. By first binding the targeted toxin to fixed cells, then binding FITC-labeled Anti-SAP to the targeted toxin, specificity can be confirmed through the use of competing molecules or a control cell line.

Saporin was used as the immunogen. The antibody was affinity-purified against saporin attached to a CnBr-Sepharose support column. The affinity-purified polyclonal antiserum was conjugated to FITC (5-iodoacetamidofluorescein) using SPDP. The conjugate binds native and recombinant saporin.

Applications include flow cytometry.

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