192-IgG Mouse Monoclonal, Cy3-labeled [FL-01]

25 ug, 100 ug

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SKU: FL-01 Categories: ,

Description

192-IgG is the antibody to the p75 neurotrophin receptor (p75NTR). The p75NTR, also known as the low affinity nerve growth factor receptor, binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NTR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death.

This fluorescent conjugate recognizes p75 receptor-positive cells in rat. It was prepared using mouse monoclonal antibody 192-IgG conjugated to Cy3. This product is routinely tested by flow cytometry.

Applications include flow cytometry, immunofluorescence, and immunocytochemistry. This product can also be used as a transport agent.

To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols

FL-01

HEK-293 cells, transfected with the p75 neurotrophin receptor were labeled with 192-IgG conjugated to Cy3 at 20 mg/ml and incubated at 37°C for 24 hours. Cells were then washed with PBS and analyzed on a fluorescent microscope under 20X magnification using a Y3 Leica filter cube.

 

 

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