Dopamine Transporter Rat Monoclonal (DAT-ECD), biotin labeled [BT-N17]


SKU: BT-N17 Category: Antibody Type: Monoclonal | Host: Rat | Reactivity: Human, rat | Conjugate: Biotin | Usage: immunoblotting, immunocytochemistry, immunohistochemistry |

The dopamine transporter is a sodium-dependent reuptake carrier that is suspected to play a role in such neurologic and psychiatric disorders as Parkinson’s disease, Tourette’s disease, schizophrenia, and addiction. It is a12-transmembrane domain transporter with the N- and C- terminus regions located within the cytoplasm. This antibody to the dopamine transporter was originally designated DAT/e2 and is now termed anti-DAT-ECD, emphasizing that it recognizes an extracellular domain.

This antibody recognizes the second extracellular loop of the dopamine transporter (DAT-ECD) in rat and human. It was produced in rat by immunization with a GST-DAT-loop fusion protein, then construction of a hybridoma with the murine nonsecreting myeloma cell line Sp2/0. The second extracellular loop, consisting of amino acids 180-218, was used to construct the fusion protein. It has been conjugated to biotin via an amide bond.

Applications include immunocytochemistry, immunohistochemistry, and immunoblotting.

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