GAD65 (B96) Human Monoclonal, biotin labeled [BT-N13]


SKU: BT-N13 Category: Antibody Type: Monoclonal | Host: Human | Reactivity: All species | Conjugate: Biotin | Usage: immunohistochemistry, immunoprecipitation, protein footprinting |

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type1) diabetes mellitus (IDDM), stiff man syndrome, and polyendocrine autoimmune disease. Autoantibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM, and thus are important markers for disease activity. These autoantibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Autoantibodies to GAD67 are found in only 15% of recent-onset IDDM patients, and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

This antibody is specific for the 65-kDa isoform of glutamic acid decarboxylase (GAD65), targeting the IDDM-E2 region (amino acids 451-570), and does not bind GAD67. To create this cell line, peripheral blood B-cells were obtained from a donor who tested positive for GAD65 autoantibodies and were immortalized with Epstein-Barr virus. It has been conjugated to biotin via an amide bond.

Applications include immunoprecipitation, immunohistochemistry, and protein footprinting. This antibody does not work in Western blotting.

To view protocol(s) for this and other products please visit:

View Data Sheet