This antibody targets conjugated Folic acid (vitamin B9). This antibody does not recognize free folic acid. Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
Using a conjugate Folic acid-Protein Carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Folic acid-BSA (1), Folinic acid-BSA (1/>900), Tetrahydrofolic acid-BSA (1/>2,000), Methotrexate-BSA (1/>50,000), Riboflavin-BSA (1/>50,000), BSA (1/>50,000).
Applications include ELISA and immunohistochemistry / immunocytochemistry.
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