Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated citrulline. This antibody does not recognize free citrulline.
Using a conjugate citrulline-glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Citrulline-G-BSA (1), Arginine-G-BSA (1/>100,000), Glutamine-G-BSA (1/>100,000), Ornithine-G-BSA (1/>100,000).
Applications include ELISA and immunohistochemistry / immunocytochemistry.
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