Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated 5-Methoxytryptophan. This antibody does not recognize free 5-Methoxytrptophan.
Using a conjugate 5-Methoxytryptophan-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: 5-Methoxytryptophan-G-BSA (1), Tryptophan-G-(Pc) (1/5,800), 5-Hydroxytryptophan-G-(Pc) (1/>50,000), 5-Methoxytryptamine-G-(Pc) (1/>50,000).
Applications include ELISA, immunocytochemistry, immunohistochemistry, immunoblotting.