Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated L-glutamate. This antibody does not recognize free L-glutamate.
Using a conjugate L-glutamate-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by experiments with the following compounds: L-Glutamate-G-BSA (1), D-Glutamate-G-(Pc) (1/>50,000), L-Aspartate-G-(Pc) (1/>50,000), D-Aspartate-G-(Pc) (1/>50,000), GABA-G-(Pc) (1/>50,000).
Applications include ELISA, immunohistochemistry, immunocytochemistry, and immunoblotting.