This antibody targets conjugated melatonin. This antibody does not recognize free melatonin. Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
Using a conjugate 5-Methoxytryptamine-Glutaric anhydride-Protein Carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: 5-Methoxytryptamine-GA-BSA (1), 5-Hydroxytryptamine-GA-BSA (1/>7,500), DL 5-Methoxytryptophan-GA-BSA (1/>9,500), Tryptamine-GA-BSA (1/>50,000), 5-Methoxytryptamine-G-BSA (1/>50,000).
Applications include ELISA.
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