Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated tyramine. This antibody does not recognize free tyramine.
Using a conjugate tyramine-glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Tyramine-G-BSA (1), Octopamine-G-(Pc) (1/800), Dopamine-G-(Pc) (1/>40,000), Tyrosine-G-(Pc) (1/>50,000).
Applications include ELISA, immunocytochemistry, immunohistochemistry, immunoblotting.