Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated tryptophan. This antibody does not recognize free tryptophan.
Using a conjugate Tryptophan-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Tryptophan-G-BSA (1), 5 Hydroxytryptophan-G-(Pc) (1/106), 5 Methoxytryptophan-G-(Pc) (1/251), Tryptophan=GA=(Pc) (1/444), Tryptophan (1/3,000), Tryptamine-G-(Pc) (1/11,000), 5 Methoxytryptamine-G-(Pc) (1/56,000), 5 Hydroxytryptamine-G-(Pc) (1/220,000).
Applications include ELISA and immunohistochemistry / immunocytochemistry.
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