Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated tyrosine. This antibody does not recognize free tyrosine.
Using a conjugate tyrosine-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Tyrosine-G-BSA (1), Phenylalanine-G-BSA (1/6,000), Tryptophan-G-BSA (1/35,000), Tyrosine-GA-BSA (1/>50,000), Tyrosine (1/>50,000).
Applications include ELISA and immunoblotting.