Monoclonal antibody to acetylcholine was obtained after AKRT mouse immunization with the conjugates: Choline-Glutaric acid-carrier proteins and hybridization of spleen cells with myeloma cell line SP2/O/Ag14. Ascites production was performed in irradiated BALB/c mice. The ascitic fluid was purified by ammonium sulfate precipitation.
This antibody targets conjugated choline-glutaric acid. This antibody does not recognize free choline-glutaric acid.
Using a conjugate choline-glutaric acid-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Choline-GA-BSA (1), Choline-AA-BSA (1/500), Choline-SA-BSA (1/2,500), Farnesol-BSA (1/>50,000), Retinoic acid-BSA (1/>50,000), Myristic acid-BSA (1/>50,000), Acetylcholine (1/>50,000), Choline (1/>50,000).
Applications include ELISA , immunocytochemistry, immunohistochemistry, and immunoblotting.
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