Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated histamine. This antibody does not recognize free histamine.
Using a conjugate Histamine-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Histamine-G-BSA (1), 1-Methylhistamine-G-BSA (1/>50,000), 3-Methylhistamine-G-BSA (1/>50,000), Histidine-G-BSA (1/>50,000).
Tested applications include immunocytochemistry and immunohistochemistry.