The dopamine transporter (DAT) is a sodium-dependent reuptake carrier that is suspected to play a role in such neurologic and psychiatric disorders as Parkinson’s disease, Tourette’s disease, schizophrenia, and addiction. It is a 12-transmembrane domain transporter with the N- and C- terminus regions located within the cytoplasm.
This antibody recognizes the N-terminus of the dopamine transporter (DAT-NT) in rat and human. It was produced in rat by immunization with a GST-DAT-NT fusion protein, then construction of a hybridoma with the murine nonsecreting myeloma cell line Sp2/0. The N-terminus, consisting of amino acids 1-66, was used to construct the fusion protein. The antibody was purified by protein A column chromatography.
Applications include immunocytochemistry, immunohistochemistry, and immunoblotting.
keywords: dopamine transporter, dat, dat-nt, n-terminus, Anti-DAT-NT, Anti-Dopamine Transporter, Parkinson’s disease, PD, brain, neuroscience
Dopamine transporter-immunoreactive neurons decrease with age in the human substantia nigra.
Ma SY, Ciliax BJ, Stebbins G, Jaffar S, Joyce JN, Cochran EJ, Kordower JH, Mash DC, Levey AI, Mufson EJ (1999) Dopamine transporter-immunoreactive neurons decrease with age in the human substantia nigra. J Comp Neurol 409(1):25-37. doi: 10.1002/(sici)1096-9861(19990621)409:13.0.co;2-e PMID: 10363709
Objective: To quantify the number of DAT immunostained neurons.
Summary: Findings demonstrate an age-related reduction in the number of substantia nigra DAT-immunoreactive neurons.
Usage: IHC (1:500)
Related Products: Dopamine Transporter Rat Monoclonal (DAT-ECD) (Cat. #AB-N17), Dopamine Transporter Rat Monoclonal (DAT-NT) (Cat. #AB-N18)
Dopamine axon varicosities in the prelimbic division of the rat prefrontal cortex exhibit sparse immunoreactivity for the dopamine transporter.
Sesack SR, Hawrylak VA, Matus C, Guido MA, Levey AI (1998) Dopamine axon varicosities in the prelimbic division of the rat prefrontal cortex exhibit sparse immunoreactivity for the dopamine transporter. J Neurosci 18(7):2697-2708. doi: 10.1523/JNEUROSCI.18-07-02697.1998 PMID: 9502827
Objective: To determine whether the reportedly greater diffusion of dopamine in the rat prefrontal cortex (PFC) as compared with the striatum is associated with a more restricted axonal distribution of the cortical DAT protein.
Summary: Results suggest that considerable extracellular diffusion of dopamine in the prelimbic PFC may result, at least in part, from a paucity of DAT content in mesocortical dopamine axons, as well as a distribution of the DAT protein at a distance from synaptic release sites. Also, different populations of dopamine neurons may selectively target the DAT to different subcellular locations.
Usage: electron microscopy
Related Products: Dopamine Transporter Rat Monoclonal (DAT-NT) (Cat. #AB-N18)
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