Poly histidine (HHHHHH) is frequently used as an affinity tag to facilitate the detection, isolation, and localization of recombinant proteins.
This high-affinity and specific histidine antibody is developed using HHHHHH peptide conjugated to KLH and affinity purified with the HHHHHH peptide on agarose. It is conjugated to horseradish peroxidase (HRP). The antibody recognizes His-tag proteins in all species.
Reported to be effective for detection, isolation and localization of His-tag proteins, ELISA, and immunoblotting. It could be utilized for direct blotting without additional use of secondary antibodies.
To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols