d-biotin is a natural vitamin that binds to avidin strongly and irreversibly.
This antibody was developed and affinity-purified with d-biotin-agarose beads using d-biotin as antigen. The antibody binds biotin strongly but reversibly. It will react with free biotin and biotinylated antibody and proteins.
Reported to be effective for direct, competitive ELISA for quantitation of vitamin biotin, western-blot analysis of the biotinylated antibodies and proteins, immunoprecipitation, immunohistochemistry, and immunochemistry.
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