Pan-specific for phosphotyrosine, but does not cross-react with phospho-serine or -threonine. Affinity-purified with phosphotyrosine immobilized on agarose followed by immune affinity adsorption with tyrosine on agarose. Conjugated to horseradish peroxidase (HRP) via reductive amination. The concentration is 250 åµg/ml.
Reported to be effective for ELISA, immunoblotting, and tyrosine kinase assay.
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