Pan-specific for phosphothreonine, slightly cross-reacts with phosphoserine, but does not cross-react with phosphotyrosine. Affinity-purified with phosphothreonine immobilized conjugated to agarose. Conjugated to d-biotin via acylation of the primary amine with active ester of biotin (NHS-activated d-biotin). The concentration is 250 åµg/ml.
Reported to be effective for ELISA and immunoblotting.
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