Recognizes proteins methylated on lysine residues (mono and di-methylysine). Does not cross-react with acetylated proteins. Affinity-purified using N-methyl (epsilon amino group) lysine conjugated to agarose as affinity matrix. Conjugated to horseradish peroxidase (HRP) via reductive amination. The concentration is 250 åµg/ml.
Reported to be effective for ELISA, immunoblotting (western blot), and immunohistochemistry.
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