Recognizes proteins methylated on lysine residues (mono and di-methyllysine). Does not cross-react with acetylated proteins. Cross-reactivity with tri-methyllysine is not tested. Affinity-purified using Ne-monomethyllysine and dimethyllysine conjugated to agarose as affinity matrix. Conjugated to d-biotin via acylation. The concentration is 250 åµg/ml.
Reported to be effective for ELISA and immunoblotting.
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