Targeting Tools: IB4-SAP

IB4, or the B4 lectin isoform from Bandeiraea simplicifolia (Griffonia simplicifolia), has played an important role in the delineation of the pathways of pain transmission. One of the two major groups of primary afferents that target the spinal cord dorsal horn neurons are labeled by IB4; the other group by TrkA and peptides such as […]

Targeting Talk: In Vivo Use of Targeted Toxins

Can you use targeted toxins in vivo? Yes, Molecular Neurosurgery is designed as a tool for in vivo use. How do you recommend administration of the targeted toxin? There are several ways to administer the toxins depending on the cells being targeted: Direct intraparenchymal pressure microinjection can be used to deliver the targeted toxin directly […]

Targeting Topics 01q1

Loss of nerve: a molecular approach to better treatment of chronic pain. Friedrich MJ. JAMA 283(2):187-188, 2000. PMID: 10634324 The use of SP-SAP (Cat. #IT-07) as a promising new method for chronic pain relief is discussed in this review article. Chronic pain has classically been treated in ways that frequently have adverse effects on the […]

Cover Article: Immunolesioning – From Spinal Cord to Brain

Contributed by Dr. Ann Schreihofer, University of Virginia, contributes this issue’s article from the laboratories of ATS customers. Dr. Schreihofer summarizes her research with anti-DBH-SAP (Cat. #IT-03) to immunolesion specific rostral ventral medulla neurons that project to the spinal cord by injection of anti-DBH-SAP into the rat spinal cord. The toxin is taken up and […]

SFN Poster of the Year 2000

Awarded by ATS at Society for Neuroscience (SFN) New Orleans, LA • November 4-9, 2000 389.13 Focal Hippocampal Hyperexcitability After Focal Interneuron Ablation In The Rat By Substance P-Saporin. J.L. Martin; R.S. Sloviter*, U. Arizona Col. of Med., Tucson, AZ, USA featuring IT-11 SSP-SAP (Poster) Hyperexcitability after prolonged seizures or head trauma may result from […]

Abstracts from Society for Neuroscience (SFN) – New Orleans, LA | November 4-9, 2000

41.7 IMMUNOHISTOCHEMICAL DETECTION OF ALPHA-7 NICOTINIC RECEPTOR EXPRESSION BY TWO DISTINCT CELL TYPES IN THE DORSAL RAPHE AND LOCUS COERULEUS OF RAT. R.S. Bitner*; A.L. Nikkel; M.W. Decker featuring IT-03 Anti-DBH-SAP (Poster) 51.22 SENSORY TRAINING IMPROVES THE ABILITY TO PROCESS STIMULI IN BARREL CORTEX AFTER BASAL FOREBRAIN LESION. O. Rahimi*; D. Tatham; S.L. Juliano featuring […]

Targeting Trends Newsletter 00q4

Below are links to view the quarterly newsletter Targeting Trends. If you would like to be added to the mailing list, please complete the information on our contact page. Newsletter Highlights Toxicology/Safety studies with SP-SAP to begin (page 1-2) Second immunotoxins: use your monoclonal or polyclonal to target cells (page 5) Mouse p75 immunotoxin to be […]

Targeting Tools: ME20.4 Monoclonal and Immunotoxin

The ME20.4 monoclonal antibody (Cat. #AB-N07) was derived from immunization of mice with WM245 melanoma cells. The antibody can be used in Western blot, immunoprecipitation, immunohistochemistry, FACS analysis, and electron microscopy. The known species reactivity includes human, primate, rabbit, raccoon, dog, cat, and sheep. The ME20.4-SAP immunotoxin (Cat. #IT-15) is a chemical conjugation between the […]

Targeting Tools: Mouse-Specific p75 Immunotoxin

The long-sought mouse cholinergic toxin has been found! The mouse cholinergic immunotoxin (Cat. #IT-16) is being released for sale at the Society for Neuroscience meeting in New Orleans, November 4-9, 2000! For years ATS has received requests for a molecule that would eliminate cholinergic neurons in the basal forebrain of mice in a manner that […]

Targeting Talk: Second Immunotoxins

What is a second immunotoxin? ATS’s second immunotoxins are conjugations of a secondary antibody (as of December 2000, either goat anti-mouse IgG or goat anti-rabbit IgG) to the ribosome-inactivating protein, saporin. How does a second immunotoxin target? The second immunotoxin uses the secondary antibody to “piggyback” onto your primary antibody in order to evaluate the […]