Wang H, Zhang L, Ye C, Li X, Sun M (2026) Intestinal epithelial ion channel TMEM16A deficiency impairs enteroendocrine cell differentiation and epithelial homeostasis and enhances anxiety-like behavior in mice. J Army Med Univ 48(15):2151-2163. doi: 10.16016/j.2097-0927.202605055
Objective: To investigate the effects of intestinal epithelial transmembrane member 16A (TMEM16A) deficiency on the differentiation phenotype of chromogranin A (CgA)-positive EECs, related gut-brain signaling molecules, hippocampal inflammatory signals and anxiety-like behavior, and to analyze the role of the vagal afferent pathway in this process
Summary: Intestinal epithelial TMEM16A deficiency attenuates the differentiation phenotype of CgA+ EECs, reduces 5-HT, TPH1, GLP-1 and PYY levels, and is accompanied by enhanced NLRP3/IL-1β inflammatory signals in the hippocampal CA3 region and anxiety-like behavior. The CCK-sensitive vagal afferent pathway is involved in TMEM16A deficiency-related anxiety-like behavior.
Usage: The wild type and knockout mice were treated with CCK-SAP (IT-31) or Blank-SAP (IT-21). Immunohistochemical staining and Western blotting were performed to detect the expression of intestinal epithelial renewal-related molecules and those in inflammatory signals in the hippocampal CA3 region.
Related Products: CCK-SAP (Cat. #IT-31), Blank-SAP (Cat. #IT-21)