Targeting Talk

Targeting Talk: Toxin Safety

Q: Your recent issue of Targeting Trends stated that it was unlikely that saporin compounds or constituents would be excreted in urine or feces. However, you acknowledge that experimental data is lacking. Have there been any tests of animal urine or feces for saporin content? My animal care staff are concerned. A: One of the …

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Targeting Talk: Toxin Safety

Safety Instructions Good laboratory technique must be employed for the safe handling of this product. This requires observation of the following practices: Wear appropriate laboratory attire, including lab coat, gloves and safety glasses. Do not pipet by mouth, inhale, ingest or allow product to come into contact with open wounds. Wash thoroughly any part of …

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Targeting Talk: Suicide Transport and Immunolesioning

What is immunolesioning? Immunolesioning is a technique for making highly selective cellular lesions using immunotoxins. The immunotoxins consist of a monoclonal antibody to a cell surface molecule and a toxic effect or moiety such as saporin, a ribosome-inactivating protein. The selectivity of the lesion made with this technique depends on the selective expression of the …

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Targeting Talk: Second Immunotoxins

What is a second immunotoxin? ATS’s second immunotoxins are conjugations of a secondary antibody (as of December 2000, either goat anti-mouse IgG or goat anti-rabbit IgG) to the ribosome-inactivating protein, saporin. How does a second immunotoxin target? The second immunotoxin uses the secondary antibody to “piggyback” onto your primary antibody in order to evaluate the …

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