Targeting Talk

Targeting Talk: Anti-DBH-SAP specificity

Q: I’m interested in your anti-DBH-saporin toxin for lesioning central catecholaminergic neurons. I see from the product description that the antibody used is a mouse monoclonal — designed to specifically target rat DBH. My interest is to produce targeted lesions in mouse transgenic. Will this product still work specifically? Thanks. A: Unfortunately, we do not …

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Targeting Talk: Flow Cytometry Services

Q: Recently we used your flow cytometry services (Cytometry Research, ATS subsidiary). Based on post flow data analysis needs, I am providing the assay group list below (withheld for confidentiality). A: Yes, all these antibodies meet the requirement of being excited by our 488nm laser, however the specific combinations you list use fluorescent probes that …

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Targeting Talk: Conjugate Calculations

I ordered a control conjugate to use alongside my targeted conjugate, but the two products are at different concentrations. How much control conjugate should I use? Conjugate products are often of differing protein concentrations, meaning dilution of one is usually necessary to ensure comparable amounts of control conjugate and targeted conjugate are used. This adjustment …

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Targeting Talk: ZAP Kit Sizes

I have been using your ZAP Antibody Internalization Kit. It is working well for me, but I can only test one antibody at a time. Do you offer the ZAP kit in larger sizes? We do offer kits with sufficient components to test multiple antibody candidates. We offer "Z4" and "Z10" sizes of kits that …

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Targeting Talk: Time Course

How long does it take to see the cell death occurring from the use of targeted toxins using saporin? Is there a time course of hours or days? The figure below illustrates the time course of cell death very effectively. Internalization and cytotoxicity of SP-SAP in primary cultures of neonatal spinal cord neurons (11). Confocal …

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Targeting Talk: conjugated Lauric Acid antibody

Does AB-T183 (Lauric Acid Rat Polyclonal, Conjugated) recognize lauric acid alone, or does it need to be conjugated to something (a protein carrier)? This antibody targets conjugated Lauric Acid. It does not recognize free lauric acid. Antisera was preabsorbed on protein carriers and ammonium sulfate-purified. Using a conjugate Lauric acid-Gluteraldehyde-Protein Carrier (PC), antibody specificity was …

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