Q: Your targeted toxin kits come with different controls. I’m not sure of the best way to use them; there is included unconjugated antibody, unconjugated saporin, and a control conjugate, mouse IgG-SAP. Should I use them all in the same experiment or for different purposes? A: For mouse IgG-containing conjugates, the ideal control is Mouse …
Advanced Targeting Systems offers a full line of antibodies to neurotransmitters; everything from serotonin and L-DOPA, to noradrenaline and dopamine. These antibodies are an excellent choice for immunohistochemistry applications as they are ideally primed for use in tissues that have been perfused with a mixture of paraformaldehyde and gluteraldehyde. Some frequently asked questions: Q: Could you …
Q: I’ve been looking at your secondary conjugates and want to see if my targeting agent is specific to certain cells. Which secondary conjugate should I use? A: It depends on two factors: 1) the type of assay you want to use, and 2) the kind of targeting agent you want to use. For in …
Over the years, ATS has frequently been asked about Saporin’s safety for use in the lab as well as when used clinically. Residual awareness of alternate Ribosome-Inactivating Proteins (RIPs) and ‘toxins’ such as Ricin have caused some researchers new to the use of RIPs to question the belief that Saporin is safe. Unlike Type 2 …
Q: Our QA group wants to know about the safety of the toxin in your conjugates? What precautions should we take in handling saporin products? A: Saporin is a Type 1 ribosome-inactivating protein (RIP), due to its N-glycosidase activity, from the seeds of Saponaria officinalis. It was first described by Fiorenzo Stirpe and his colleagues …
Q: I’m trying to find out if enough Anti-DBH-SAP will be retrogradely transported and taken up by non targeted sympathetic neurons by bulk fluid-phase endocytosis. Does saporin become degraded after it kills the neuron or does it enter the extracellular matrix? A: It is very unlikely that a targeted toxin such as Anti-DBH-SAP is freed …
Q: Hello, I have used your Anti-DBH-SAP (Cat. #IT-03) conjugate, and I’m having a hard time finding this citation: R.G. Wiley, D.A. Lappi. Suicide Transport and Immunolesioning. Molecular Biology Intelligence Unit, R.G. Landes Co, Austin, TX (1994). Do you know where I could find a copy? A: This book is available in many university libraries …
Targeting Talk: Suicide Transport and Immunolesioning Book Read More »
Q: For in vitro cytotoxicity assays, could you tell me: 1) whether you incubate primary with your Saporin secondary for a specific amount of time prior to cell addition, and 2) do you use a single concentration of secondary per well or a primary:secondary ratio — like 1:2 or 1:4? A: The primary antibody should …
Q: Your targeted toxin kits come with different controls. I’m not sure of the best way to use them; there is included unconjugated antibody, unconjugated saporin, and a control conjugate, mouse IgG-SAP. Should I use them all in the same experiment or for different purposes? A: For mouse IgG-containing conjugates, the ideal control is Mouse …
Q: I ordered a targeted toxin. Will it come in powder form? How do I re-dissolve it? A: Our Saporin conjugate products are all provided in sterile PBS solution within a concentration range of 0.5 – 3 mg/ml. Saporin is an extremely safe ‘toxin’ to handle in standard laboratory environments when in solution for several …