192-IgG-SAP and the Sleep Regulatory System

Acute Effects of Alcohol on Sleep Are Mediated by Components of Homeostatic Sleep Regulatory System: An Editorial Highlight for ‘Lesions of the Basal Forebrain Cholinergic Neurons Attenuates Sleepiness and Adenosine after Alcohol Consumption.’ Alam, MN, & McGinty, D. (2017). J Neurochem 2017/07/25.

In the study, titled ‘Lesions of the basal forebrain cholinergic neurons attenuates sleepiness and adenosine after alcohol consumption’ Sharma and colleagues (1) report that the wake-promoting BF cholinergic neurons are critically involved in the acute alcohol-induced sleep promoting response and that extracellular adenosine buildup in the BF mediates this response.

Using 192-IgG-SAP (Cat. #IT-01), they ablated BF cholinergic neurons unilaterally and compared extracellular adenosine levels on lesioned versus non-lesioned sides after local delivery of alcohol via reverse microdialysis. They found that adenosine levels were significantly lower (nearly 50%) on the side with a loss of cholinergic neurons.

  1. Lesions of the Basal Forebrain Cholinergic Neurons Attenuates Sleepiness and Adenosine after Alcohol Consumption. Sharma, R, Sahota, P, & Thakkar, MM. (2017). J Neurochem 2017/04/27.

192-IgG-SAP (Cat. #IT-01)

Intraventricular injection of 192-IgG-SAP (192-Saporin) results in almost complete elimination of LNGFR (p75NTR)-positive cells in rat. 192-IgG-SAP is directed to a cell-surface antigen that is only expressed at high levels on neurons in the cholinergic basal forebrain (CBF). The antigen, p75NTR, is not expressed on the neighboring, non-cholinergic neurons. 192-IgG-SAP specifically eliminates cholinergic neurons of the basal forebrain, medial septum, diagonal band of Broca, nucleus basalis of Meynert, and Purkinje neurons of the cerebellum.

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