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Targeting Talk: Using Kit Controls

Q: Your targeted toxin kits come with different controls. I’m not sure of the best way to use them; there is included unconjugated antibody, unconjugated saporin, and a control conjugate, mouse IgG-SAP. Should I use them all in the same experiment or for different purposes? A: For mouse IgG-containing conjugates, the ideal control is Mouse […]

Targeting Talk: Neurotransmitter Antibodies

Advanced Targeting Systems offers a full line of antibodies to neurotransmitters; everything from serotonin and L-DOPA, to noradrenaline and dopamine. These antibodies are an excellent choice for immunohistochemistry applications as they are ideally primed for use in tissues that have been perfused with a mixture of paraformaldehyde and gluteraldehyde. Some frequently asked questions: Q:  Could you […]

New Product Manager Alena Bishop

She’s here to tell you, in her own words, what she is working on and what products to look forward to: “One of the projects I have been working on involves a novel use of specific strains of Clostridium botulinum that inhibits cells by blocking the release of neurotransmitters. This effect is long-lasting but not […]

Targeting Talk: Streptavidin-ZAP Applications

Q: I’ve been looking at your secondary conjugates and want to see if my targeting agent is specific to certain cells. Which secondary conjugate should I use? A: It depends on two factors: 1) the type of assay you want to use, and 2) the kind of targeting agent you want to use. For in […]

Targeting Talk: Saporin Safety

Over the years, ATS has frequently been confronted with questions about Saporin’s safety for use in the lab as well as when used clinically. Residual awareness of alternate Ribosome-Inactivating Proteins (RIPs) and ‘toxins’ such as Ricin have poisoned the belief that Saporin is safe. As a Type I RIP, Saporin has no binding chain and […]

Targeting Talk: Safety of the Toxin

Q: Our QA group wants to know about the safety of the toxin in your conjugates? What precautions should we take in handling saporin products? A: Saporin is a Type 1 ribosome-inactivating protein (RIP), due to its N-glycosidase activity, from the seeds of Saponaria officinalis. It was first described by Fiorenzo Stirpe and his colleagues […]

Targeting Talk: Retrograde Transport

Q: I’m trying to find out if enough Anti-DBH-SAP will be retrogradely transported and taken up by non targeted sympathetic neurons by bulk fluid-phase endocytosis. Does saporin become degraded after it kills the neuron or does it enter the extracellular matrix? A: It is very unlikely that a targeted toxin such as Anti-DBH-SAP is freed […]

Targeting Talk: Suicide Transport and Immunolesioning Book

Q: Hello, I have used your Anti-DBH-SAP (Cat. #IT-03) conjugate, and I’m having a hard time finding this citation: R.G. Wiley, D.A. Lappi. Suicide Transport and Immunolesioning. Molecular Biology Intelligence Unit, R.G. Landes Co, Austin, TX (1994). Do you know where I could find a copy? A: This book is available in many university libraries […]

Targeting Talk: in vitro cytotoxicity assays

Q: For in vitro cytotoxicity assays, could you tell me: 1) whether you incubate primary with your Saporin secondary for a specific amount of time prior to cell addition, and 2) do you use a single concentration of secondary per well or a primary:secondary ratio — like 1:2 or 1:4? A: The primary antibody should […]

Targeting Talk: Using Kit Controls

Q: Your targeted toxin kits come with different controls. I’m not sure of the best way to use them; there is included unconjugated antibody, unconjugated saporin, and a control conjugate, mouse IgG-SAP. Should I use them all in the same experiment or for different purposes? A: For mouse IgG-containing conjugates, the ideal control is Mouse […]